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Scholars

John Chorba, MD

John Chorba, MD

University of California, San Francisco

Disease Area

Cardiovascular


Focus

Inhibiting PCSK9 Self-Proteolysis to Treat Atherosclerosis


Scholar Profile

2021 Harrington Scholar-Innovator

Atherosclerosis—hardening and narrowing of artery walls caused by a buildup of cholesterol plaques—is a major risk factor for cardiovascular disease and stroke, the leading causes of death in the United States. A primary driver of atherosclerosis is elevated levels of low density lipoprotein (LDL), sometimes called ‘bad cholesterol,' in the blood, and the protoprotein PCSK9 is a contributor to elevated LDL levels. PCSK9 downregulates the hepatic LDL receptor, which clears LDL from the bloodstream. This downregulation raises LDL levels and accelerates atherosclerosis.

Dr. Chorba and his colleagues are pursuing a small molecule approach to inhibit the action of PCSK9 but are challenged by the fact that its biochemical output is difficult to study. Proteolysis (cleaving or breaking down of a protein) is required for PCSK9's effect on the LDL receptor. However, as a single-turnover protease, PCSK9 cleaves only once; afterwards there is no further readout of its protease activity.

Dr. Chorba and his colleagues have developed a cell-based method to evaluate PCSK9's proteolytic activity. They created a 70,000 compound high-throughput screen resulting in hit compounds that act as inhibitors of PCSK9's proteolytic function. The researchers theorize that the development of a lead compound acting by its this novel mechanism of action will increase hepatic LDL receptor function and lower serum LDL.

“I think there are more mechanisms we can use to target PCSK9 than were previously thought,” Dr. Chorba says. “There are individuals who have mutations in PCSK9 where their PCSK9 simply doesn't work. Our strategy, at least in theory, would mirror some of those mutations.”